Coding

Part:BBa_K2352016:Design

Designed by: Hannah Schmidt   Group: iGEM17_UFlorida   (2017-10-25)


ARO10 2-oxo acid decarboxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 51


Design Notes

The sequence had to be optimized for expression in E. coli. The sequence also had to be made compatible for iGEM RFC10.


Source

The sequence for this part was sourced from the Saccharomyces genome database. The sequence was then optimized for expression in E. coli and made compatible with RFC10 before it was sequenced by IDT.

References